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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has been observed in many tumors, which results in chromosome instability, and so could lead to tumorigenesis. This protein was uncovered to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a vital function in promoting mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are explained. Two further splice variants were noted but in depth nucleotide sequence information will not be still available.

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Blocking peptides are peptides that bind especially into the goal antibody and block antibody binding. These peptide commonly contains the epitope acknowledged through the antibody.

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This information outlines even more attainable optimization of cellular and immunoassay parameters to make sure the best possible results are...

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This guide outlines additional doable optimization of cellular and immunoassay parameters to be sure the very best success are...

Antibodies bound to the blocking peptide not bind on the epitope to the focus on protein. By evaluating the staining within the blocked antibody compared to the antibody on your own, one can see which staining is specific.

Concentrate on Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinct expression and degradation patterns which contribute to your temporal coordination of every mitotic celebration. Cyclin E types a posh with and functions for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and it is degraded as cells development by means of S phase.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...

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